Journal: Journal of Clinical Microbiology

Article Title: Human Cytomegalovirus-Specific T-Cell Immune Reconstitution in Preemptively Treated Heart Transplant Recipients Identifies Subjects at Critical Risk for Infection

doi: 10.1128/JCM.06406-11

Figure Lengend Snippet: ELISPOT assay as a predictor of CMV infection. Box-and-whisker plot of CMV-specific IFN-γ ELISPOT levels of HTXs who controlled or experienced CMV viremia within 60 days after ELISPOT determination. The rectangular box connects the upper and lower quartiles and the median. Whiskers connect the upper and lower extremes. The ELISPOT level corresponds to the number of CMV pp65-specific IFN-γ spots/200,000 PBMCs. ELISPOT assay data included those for the test performed from 30 to 360 days posttransplantation. *, P < 0.05.

Article Snippet: PBMCs were resuspended in RPMI 1640 medium supplemented with 10% human type AB serum (Sigma-Aldrich) and seeded at a concentration of 1 × 10 6 cells/ml in 96-well IFN-γ-coated ELISPOT assay plates (Autoimmun Diagnostika).

Techniques: Enzyme-linked Immunospot, Infection, Whisker Assay

Journal: Journal of Clinical Microbiology

Article Title: Human Cytomegalovirus-Specific T-Cell Immune Reconstitution in Preemptively Treated Heart Transplant Recipients Identifies Subjects at Critical Risk for Infection

doi: 10.1128/JCM.06406-11

Figure Lengend Snippet: CMV-specific immunity in 10 pretransplant patients and 48 HTXs. Box-and-whiskers plot of pretransplant (control group), early (<100 days posttransplant), and late (>100 days posttransplant) CMV-specific IFN-γ ELISPOT levels in HTXs. Data are for 19 HTXs with single ELISPOT assay determinations taken <100 or >100 days after transplant and ELISPOT assay and 29 HTXs followed prospectively both before and after 100 days after transplant. Numbers in parentheses display the number of ELISPOT assay determinations done for each group.

Article Snippet: PBMCs were resuspended in RPMI 1640 medium supplemented with 10% human type AB serum (Sigma-Aldrich) and seeded at a concentration of 1 × 10 6 cells/ml in 96-well IFN-γ-coated ELISPOT assay plates (Autoimmun Diagnostika).

Techniques: Enzyme-linked Immunospot

Journal: Journal of Clinical Microbiology

Article Title: Human Cytomegalovirus-Specific T-Cell Immune Reconstitution in Preemptively Treated Heart Transplant Recipients Identifies Subjects at Critical Risk for Infection

doi: 10.1128/JCM.06406-11

Figure Lengend Snippet: Immune reconstitution of 29 HTXs followed prospectively before and after 100 days after transplant. Dotted lines show HTX IFN-γ ELISPOT levels before and after 100 days posttransplant. Arrows indicate the CMV-specific immune response of one HTX who experienced CMV disease. For the other patient experiencing CMV disease, we did not have data from sequential time points.

Article Snippet: PBMCs were resuspended in RPMI 1640 medium supplemented with 10% human type AB serum (Sigma-Aldrich) and seeded at a concentration of 1 × 10 6 cells/ml in 96-well IFN-γ-coated ELISPOT assay plates (Autoimmun Diagnostika).

Techniques: Enzyme-linked Immunospot